Antibacterial mechanism of action and in silico molecular docking studies of Cupressus funebris essential oil against drug resistant bacterial strains

The primary objective of this research work was to study the antibacterial effects Cupressus funebris essential oil (EO) against various drug resistant bacterial pathogens along with studying molecular docking interactions major components EO key proteins/enzymes. Gas chromatography-mass spectrometry used analyse chemical composition EO. initial screening performed by using disc diffusion and microdilution methods. Scanning electron microscopy also in order on cell morphology. Further, studies were Autodock Vina results visualised BIOVIA Discovery Studio. showed presence 15 citronellal, terpinene-4-ol, α-phellandrene 1,8-cineole as Results indicated that exhibited dose-dependent well time dependent effects. scanning led membrane rupture permeabilization cells. Molecular compounds (citronellal terpinene-4ol) strong active site DNA gyrase enzyme explaining mode action Ciprofloxacin for which stronger target protein than citronellal or terpinene-4-ol. In conclusion, findings current causes permeabilization, shows time-dependent action, interacting crucial viz., studies.